Electroosmotic flow in soft clay and measures to promote movement under direct current electric field.

Electroosmosis has been proposed as a technique to reduce moisture and thus increase the stability of soft clay. However, its high energy consumption and uneven reinforcement effect has limited its popularization and application in practical engineering. This paper presents the results of some electrokinetic tests performed on clayey specimens with different electrification time and anode boundary conditions. The results indicate that the timing of the formation of electroosmotic flow (EF) by the water originally contained in different soil cross sections, from the anode to the cathode, varies. The measuring soil cross section nearest the anode first reached the limiting water content of 22%±3% and electroosmosis had to be stopped. Water injection into the anode during electroosmosis enhanced further drainage of other four measuring soil cross sections until the second soil cross section from the anode reached the limiting water content of 30%±2%. Electroosmosis with water injection into the anode technique provides more uniform reinforcement, increasing EF, and environmental protection. The experimental results highlighted the relevant and expected contribution of water injection into the anode on the effectiveness of the electroosmotic treatment as a soft clay improvement technique.

Electroosmosis and electrophoresis in soft clay under direct current electric field.

The demand for innovative and cost-effective in-situ consolidation technologies in soft clay stimulated the effort to employ electrokinetic phenomena in soils under an electric field. This work explains the principles of electrophoresis and electroosmosis in soft clay under an electric field and discusses the implacations of these results for field implementation of the technique. The experimental work reported here was carried out to shed light on the principles by considering various influencing factors. The results show that there is a threshold current (about 15 mA) for electroosmosis and electrophoresis in soft clay. Below the threshold current, electrophoresis plays a major role and is accompanied by weak electroosmosis. The particle size, dry density, and boundary rise height of electrophoretic soil in anode chamber are mainly affected by soil moisture content, electrode in electrolyte length, and bottom of electrode to soil surface distance. The closer the soil moisture content is to the average value of the liquid limit and plastic limit, the denser the electrophoretic soil will be. Above the threshold current, almost only electroosmosis occurs. The greater the current, the greater the electroosmosis. In practical engineering application, electrodes are usually directly inserted into the soft clay. This results in the current usually being much greater than the threshold current, meaning the electrophoretic phenomenon in the soil can be negligible.

Application value of EV/EV71/CA16-SAT detection in children with hand-foot-mouth disease.

The objective of this work was to explore the application value of a new type of fluorescent nucleic acid isothermal amplification (SAT) to detect EV/EV71/CA16-SAT in children with hand-foot-mouth disease (HFMD). For this purpose, from March 2017 to September 2019, Chengdu Children's Specialized Hospital collected throat swabs from children with clinical manifestations of hand, foot and mouth disease, and used SAT technology to screen and detect universal enterovirus (EV) nucleic acid (There were 1860 children with EV-RNA) positive. Patients who are EV-RNA positive at any time: first use the same throat swab specimen to detect EV71/CA16-RNA; secondly, collect venous blood and use the colloidal gold method to detect IgM antibodies in EV71/CA16 serum. The patients with positive EV71/CA16-RNA or EV71/CA16-IgM (or both) were repeated the above two methods 2 weeks and 4 weeks after standard treatment for review and comprehensive analysis. Results showed that 763 cases were enrolled for the first time: 59.76% were male and 40.24% were female; the age ranged from 1 month to 13 years, of which 69.06% were from 1 to 4 years old; CA16-RNA positive 56.23%, EV71-RNA positive 21.89%, CA16/EV71 -RNA were all positive in 1.57%; CA16-IgM was positive in 64.48%, EV71-IgM was positive in 54.26%, and CA16/EV71-IgM were both positive in 18.74%. After 2 weeks, 722 cases were reexamined: 26.73% were positive for CA16-RNA, 7.89% were positive for EV71-RNA, 0.28% were both positive for CA16/EV71-RNA; 66.21% were positive for CA16-IgM, 51.52% were positive for EV71-IgM, and IgM were all positive in 17.73%. Four weeks later, 489 cases were reexamined: among them, CA16-RNA positive 5.73% of which were positive for EV71 color RNA (0.005%), and 12.68% of them were all positive for EV71lym. The strategy of combining SAT technology and colloidal gold method to detect EV/EV71/CA16 nucleic acid (RNA) and serum IgM antibody in children HFMD can improve the early detection rate and accuracy of HFMD; According to the comprehensive analysis of the detection results of children with HFMD at the early stage, 2 weeks and 4 weeks of the present study, it is suggested that EV/EV71/CA16-SAT nucleic acid detection can be used to judge the prognosis, follow-up treatment, set isolation time, return students to school, and community management in children with HFMD. and prevention and control have more clinical application value.

Engineering application of vacuum preloading combined with electroosmosis technique in excavation of soft soil on complex terrain.

This paper presents the design and construction of vacuum preloading incorporated with electroosmosis (VPE) engineering application for the treatment of soft soil on complex terrain for sluice foundation excavation in order to reduce the amount of cement used in construction. Monitoring was conducted during the VPE treatment and laboratory geotechnical tests were carried out once the treatment came to an end. Results show that the electrification mode has a significant influence on electric energy consumption. Stepped-up voltage helped in saving electric energy while electrode conversion consumed a lot of electric energy. The dispersion of soil parameters became larger after VPE treatment. The stability of physical parameters is better than the mechanical parameters, and the latter is better than the deformation parameters. Soil water content has a linear relationship with density and compression coefficient. The given linear fitting equations can help in simplifying the calculation and acquisition of these indexes. Although the mean values of the soil index parameters slightly improved, their coefficient of variation (COV) significantly increased. These locations with improved index parameters scattering in the construction site ensured that the subsequent construction tasks such as pit slope and excavation were successfully realized in this area.

Electrokinetic Remediation of Zn-Polluted Soft Clay Using a Novel Electrolyte Chamber Configuration.

This study investigated a novel electrolyte chamber configuration for heavy-metal-contaminated fine-grained soil to reduce the leakage of electrolyte solution and alleviate secondary pollution, finally promoting the electrokinetic remediation (EKR) potential to be scaled up for application. Experiments were conducted on clay spiked with Zn to investigate the feasibility of the novel EKR configuration and the effect of different electrolyte compositions on the electrokinetic remedial efficiency. The results show that the electrolyte chamber situated above the soil surface is promising for the remediation of Zn-contaminated soft clay. Using 0.2 M citric acid as the anolytes and catholytes was an excellent choice for pH control in the soil and the electrolytes. Through this, the removal efficiency in different soil sections was relatively uniform and more than 90% of the initial Zn was removed. The supplementing of electrolytes resulted in the water content in the soil being distributed evenly and finally sustained at approximately 43%. Consequently, this study proved that the novel EKR configuration is suitable for fine-grained soil contaminated with Zn.

Three-dimensional inversion analysis of transient electromagnetic response signals of water-bearing abnormal bodies in tunnels based on numerical characteristic parameters.

The transient electromagnetic inversion of detection signals mainly depends on fast inversion in the half-space state. However, the interpretation results have a certain degree of uncertainty and blindness, so the accuracy and applicability of the three-dimensional full-space inversion need to be investigated. Two different three-dimensional full-space inversions were carried out. First, the numerical characteristic parameters of the response signals were extracted. Then, the correlations between the numerical characteristic parameters and physical parameters of the water-bearing abnormal bodies were judged, and the judgment criterion of the iterative direction was proposed. Finally, the inversion methods of the iterative algorithm and the BP neural network were utilized based on the virtual example samples. The results illustrate that the proposed numerical characteristic parameters can accurately reflect the response curve of the full-space surrounding rock. The difference in the numerical characteristic parameters was used to determine the update direction and correction value. Both inversion methods have their advantages and disadvantages. A single inversion method cannot realize the three-dimensional inversion of the physical parameters of water-bearing abnormal bodies quickly, effectively and intelligently. Therefore, the benefits of different inversion methods need to be considered to comprehensively select a reasonable inversion method. The results can provide essential ideas for the subsequent interpretation of the three-dimensional spatial response signals of water-bearing abnormal bodies.

An improved Smoothed Particle Hydrodynamics (SPH) method for modelling the cracking processes of teeth and its applications.

The present work aims to propose a meshless method to establish the tooth meso-structures and model the tooth fracturing processes as well as investigate the influencing factors that affect the dental mechanical properties. To this end, the traditional kernel function in the SPH method has been improved by introducing a fracture mark ξ to realize the progressive failure processes of teeth; The "Particle Searching Method" has been proposed, which can realize the establishments of microstructures of teeth such as enamel, dentine, pulp, PDL and alvedar bones. The Weibull function is introduced to represent the heterogeneity of teeth, which can realize the random distribution characteristics of dental mechanical parameters. The simulation results of homogeneous and heterogeneous teeth show that the failure mode changes from tensile splitting (homogeneous) to shear failure (heterogeneous). Meanwhile, the fracture networks become more complex, and the failure stress decreases sharply. The cuspal angles also have a great impact on the teeth fracture characteristics. The failure modes changes from tensile splitting of the enamel tip to the cracking from the contact points between the enamel and the rigid ball; Different fssural morphologies have little influences on the teeth failure characteristics. The research results can provide some references for the applications of SPH method into biomechanical simulations such as teeth failure. Meanwhile, it can also provide some guidance for the understandings of the internal mechanisms of teeth fracture processes, the diagnosis and treatments of clinical diseased teeth as well as the design of bionic teeth materials.

Response of vertically-loaded pile in spatially-variable cement-treated soil.

Despite the extensive application prospects of piles in cement-treated soil, few studies have explored the ultimate bearing capacity especially in consideration of the spatial variability of cement-treated soil. This study examines the performance of driven piles which were installed inside the cement-treated ground, considering the inherent spatial variability of the cemented soil and the positioning error during piles installation through finite element analyses. The deterministic and random finite element analysis results have shown that the shaft resistance mainly provided the ultimate bearing resistance of piles in cement-treated soil. The spatial variability reduced the global performance of pile installed through a cement-treated soil. The ultimate bearing resistance of the pile inserted in cement-treated soil was controlled by drained condition. Drained ultimate bearing resistance should be used to determine the design working compression load of pile in cement-treated soil.

Simulating the impact of piers on hydrodynamics and pollutant transport: A case study in the Middle Yangtze River.

It is known that channel engineering, including the construction of piers, will change the river hydrodynamic characteristics, which is a significant factor affecting the transport process of pollutants. With this regard, this study uses the well-validated and tested hydrodynamic module and transport module of MIKE 21 to simulate the hydrodynamics and water quality under various pier densities in the Wuhan reach. Hydrodynamic changes around the piers show spatial differences, which are similar under different discharges. The range and amplitude of hydrodynamic spatial variations increase with the increase in pier density. However, there is a critical value of 1.25 to 2.5 units/km. When the pier density is less than this critical value, this type of cumulative effect is the most significant. Additionally, greater changes can be found in chemical oxygen demand concentrations, which also show spatial and temporal variations. The area with high chemical oxygen demand concentration upstream and downstream from the engineering area exhibits the distribution characteristics of "decrease in the downstream area and increase in the upstream area" and "increase in downstream the area and decrease in the upstream area" respectively. In the reach section of the engineering area, the area with high chemical oxygen demand concentration increases in the front area near the piers and decreases near the shoreline. Furthermore, the concentration shows attenuation actions with a longer residence time owing to the buffering effect of pier groups. These results have significant implications on shoreline planning and utilization. Moreover, they provide scientific guidelines for water management.

Response of vegetation to submergence along Jingjiang Reach of the Yangtze River.

This work explores the changes in vegetation coverage and submergence time of floodplains along the middle and lower reaches of the Yangtze River (i.e., the Jingjiang River) and the relations between them. As the Three Gorges Dam has been operating for more than 10 years, the original vegetative environment has been greatly altered in this region. The two main aspects of these changes were discovered by analyzing year-end image data from remote sensing satellites using a dimidiate pixel model, based on the normalized difference vegetation index, and by calculating water level and topographic data over a distance of 360 km from 2003-2015. Given that the channels had adjusted laterally, thus exhibiting deeper and broader geometries due to the Three Gorges Dam, 11 floodplains were classified into three groups with distinctive features. The evidence shows that, the floodplains with high elevation have formed steady vegetation areas and could hardly be affected by runoff and usually occupied by humans. The low elevation group has not met the minimal threshold of submerging time for vegetation growth, and no plants were observed so far. Based on the facts summed up from the floodplains with variable elevation, days needed to spot vegetation ranges from 70 to 120 days which happened typically near 2006 and between 2008 and 2010, respectively, and a negative correlation was detected between submergence time and vegetation coverage within a certain range. Thus, floods optimized by the Three Gorges Dam have directly influenced plant growth in the floodplains and may also affect our ability to manage certain types of large floods. Our conclusions may provide a basis for establishing flood criteria to manage the floodplain vegetation and evaluating possible increases in resistance caused by high-flow flooding when these floodplains are submerged.

[Influence of Meteorological Factors on HFMD and Construction of Prediction Model in Sichuan Province].

OBJECTIVE: To analyze the epidemiological characteristics of hand, foot and mouth disease (HFMD) in Sichuan Province from 2013 to 2019. To study the correlation between meteorological factors and the incidence of HFMD and construct a prediction model. METHODS: The HMFD surveillance data and meteorological data from 2013 to 2019 in Sichuan Province were collected through the Chinese Center for Disease Control and Prevention and the China Meteorological data Network. Spearman correlation was used to analyze the relationship between HFMD incidence and meteorological factors. Multiple regression model and support vector regression (SVR) model were used to construct HFMD incidence prediction models respectively. RESULTS: A total of 615 840 cases of HFMD and 81 deaths were reported from 2013 to 2019. The average annual incidence rate was 107.31/105, and the mortality rate was 0.16/106. Spearman correlation analysis showed that the monthly incidence rate of HFMD was correlated with monthly average relative humidity (r=0.342), monthly average temperature (r=0.284), monthly average water vapor pressure (r=0.304) and monthly average days of precipitation (r=0.259). The prediction effect of the SVR model (R2=0.836) was better than the multiple regression model (R2=0.375). The SVR model provided a good fit to the monthly incidence of HFMD from 2013 to 2018, and can predict the peak incidence of HFMD in 2019. CONCLUSION: Relative humidity has the greatest influence on the incidence of HFMD. The fitting value of SVR model is in good agreement with the actual value, which is valuable in predicting the incidence of HFMD in Sichuan Province.

An Automatic Stationary Water Color Parameters Observation System for Shallow Waters: Designment and Applications.

Measurements of the above-water spectrum and concerned water color parameters (WCPs) are crucial for research and applications in water environment remote sensing. Due to the lack of system integration and automatization, conventional methods are labor-intensive, time-consuming, and prone to subjective influences. To obtain a highly accurate and long-term consistent spectrum and concurrent WCPs (Chl-a (chlorophyll-a), turbidity, and CDOM (Colored Dissolved Organic Matter)) data with a relatively low cost, an Automatic Stationary Water Color Parameters Observation System (AFWCPOS) was developed. Controlled by an automatic platform, the spectral and WCPs data were collected by TriOS RAMSES hyperspectral spectroradiometers and WETLabs ECO (Environmental Characterization Optics) fluorometers following the measurement protocol. Experiment and initial validations of AFWCPOS were carried out in Poyang Lake, the largest freshwater lake in China, from 20 to 28 July 2013. Results proved that the spectral data from AFWCPOS were highly consistent with the commonly used portable SVC (Spectra Vista Corporation) HR-1024 field spectroradiometer, with the coefficient of determination (R2) of 0.96, unbiased percent difference (UPD) of 0.14, and mean relative difference (MRD) of 0.078. With advantages of continuous and high degrees of automation monitoring, the AFWCPOS has great potential in capture diurnal and inter-diurnal variations in the test site of Poyang Lake, as well as another high-dynamic shallow coastal and inland waters, which will benefit routine water quality monitoring with high quality and high-frequency time-series observations. In addition, a successful case based on Landsat 8 OLI (Operational Land Imager) image and in-situ data collected by AFWCPOS showed it's potential in remote sensing applications. The spatial distribution of Chl-a, turbidity, and CDOM were mapped, which were explainable and similar to previous researches. These results showed our system was able to obtain reliable and valuable data for water environment monitoring.

Temporal Variation of Chlorophyll-a Concentrations in Highly Dynamic Waters from Unattended Sensors and Remote Sensing Observations.

Monitoring of water quality changes in highly dynamic inland lakes is frequently impeded by insufficient spatial and temporal coverage, for both field surveys and remote sensing methods. To track short-term variations of chlorophyll fluorescence and chlorophyll-a concentrations in Poyang Lake, the largest freshwater lake in China, high-frequency, in-situ, measurements were collected from two fixed stations. The K-mean clustering method was also applied to identify clusters with similar spatio-temporal variations, using remote sensing Chl-a data products from the MERIS satellite, taken from 2003 to 2012. Four lake area classes were obtained with distinct spatio-temporal patterns, two of which were selected for in situ measurement. Distinct daily periodic variations were observed, with peaks at approximately 3:00 PM and troughs at night or early morning. Short-term variations of chlorophyll fluorescence and Chl-a levels were revealed, with a maximum intra-diurnal ratio of 5.1 and inter-diurnal ratio of 7.4, respectively. Using geostatistical analysis, the temporal range of chlorophyll fluorescence and corresponding Chl-a variations was determined to be 9.6 h, which indicates that there is a temporal discrepancy between Chl-a variations and the sampling frequency of current satellite missions. An analysis of the optimal sampling strategies demonstrated that the influence of the sampling time on the mean Chl-a concentrations observed was higher than 25%, and the uncertainty of any single Terra/MODIS or Aqua/MODIS observation was approximately 15%. Therefore, sampling twice a day is essential to resolve Chl-a variations with a bias level of 10% or less. The results highlight short-term variations of critical water quality parameters in freshwater, and they help identify specific design requirements for geostationary earth observation missions, so that they can better address the challenges of monitoring complex coastal and inland environments around the world.

Estimation of suspended particulate matter in turbid coastal waters: application to hyperspectral satellite imagery.

An empirical algorithm is proposed to estimate suspended particulate matter (SPM) ranging from 0.675 to 25.7 mg L-1 in the turbid Pearl River estuary (PRE). Comparisons between model predicted and in situ measured SPM resulted in R2s of 0.97 and 0.88 and mean absolute percentage errors (MAPEs) of 23.96% and 29.69% by using the calibration and validation data sets, respectively. The developed algorithm demonstrated the highest accuracy when compared with existing ones for turbid coastal waters. The diurnal dynamics of SPM was revealed by applying the proposed algorithm to reflectance data collected by a moored buoy in the PRE. The established algorithm was implemented to Hyperspectral Imager for the Coastal Ocean (HICO) data and the distribution pattern of SPM in the PRE was elucidated. Validation of HICO-derived reflectance data by using concurrent MODIS/Aqua data as a benchmark indicated their reliability. Factors influencing variability of SPM in the PRE were analyzed, which implicated the combined effects of wind, tide, rainfall, and circulation as the cause.

Novel method for quantifying the cell size of marine phytoplankton based on optical measurements.

Phytoplankton size is important for the pelagic food web and oceanic ecosystems. However, the size of phytoplankton is difficult to quantify because of methodological constraints. To address this limitation, we have exploited the phytoplankton package effect to develop a new method for estimating the mean cell size of individual phytoplankton populations. This method was validated using a data set that contained simultaneous measurements of phytoplankton absorption and cell size distributions from 13 phytoplankton species. Comparing with existing methods, our method is more efficient with good accuracy, and it could potentially be applied in current in situ optical instruments.

[Construction and application of an onboard absorption analyzer device for CDOM].

Colored dissolved organic matter (CDOM) plays an important role in marine ecosystems. In order to solve the current problems in measurement of CDOM absorption, an automated onboard analyzer based on liquid core waveguides (Teflon AF LWCC/LCW) was constructed. This analyzer has remarkable characteristics including adjusted optical pathlength, wide measurement range, and high sensitivity. The model of filtration and injection can implement the function of automated filtration, sample injection, and LWCC cleaning. The LabVIEW software platform can efficiently control the running state of the analyzer and acquire real time data including light absorption spectra, GPS data, and CTW data. By the comparison experiments and shipboard measurements, it was proved that the analyzer was reliable and robust.

Variations in the optical scattering properties of phytoplankton cultures.

The scattering and backscattering coefficients of 15 phytoplankton species were determined in the laboratory using the acs and BB9 instruments. The spectral variability of scattering properties was investigated and the homogenous sphere model based on Mie theory was also evaluated. The scattering efficiencies at 510 nm varied from 1.42 to 2.26, and the backscattering efficiencies varied from 0.003 to 0.020. The backscattering ratios at 510 nm varied from 0.17% to 0.97%, with a mean value of 0.58%. The scattering properties were influenced by algal cell size and cellular particulate organic carbon content rather than the chlorophyll a concentration. Comparison of the measured results to the values estimated using the homogenous sphere model showed that: (1) The model could well reproduce the spectral scattering coefficient with relative deviations of 5-39%, which indicates that cell shape and internal structure have no significant effects on predicting the scattering spectra; (2) Although the homogenous sphere model generally reflected the spectral trend of backscattering spectra for most species, it severely underestimated the backscattering coefficients by 1.4-48.6 folds at 510 nm. The deviations for Chaetoceros sp. and Microcystis aeruginosa were large and might be due to algal cell chain links and intracellular gas vacuoles, respectively.

[Variations in the optical absorption and attenuation properties of cultured phytoplankton and their relationships with cell size].

The spectral absorption and attenuation coefficients of 16 phytoplankton species were measured in the laboratory using acs instrument. Ancillary measurements included particle size distribution and chlorophyll a concentration (Chl a). The results indicated that both algal cell size and Chl a were the two major factors dominating the magnitudes of the spectral absorption and attenuation coefficients. The spectral behaviors of attenuation spectra were dominated by algal cell size, the relationship of them didn't follow the monotonic function. Both the ratio of absorption in blue and red waveband and the spectral slope of absorption coefficient were influenced by the product of algal cell density and squares of cell size rather than algal cell size alone. The relationship between algal cell size and both absorption and attenuation spectra would be interpreted by Mie theory for homogenous sphere, which imply that the heterogeneity and non-spherical shape in algal cell morphology and internal structure have little effect on the inherent association among them.

Variation of particulate organic carbon and its relationship with bio-optical properties during a phytoplankton bloom in the Pearl River estuary.

In this study, variations in the particulate organic carbon (POC) were monitored during a phytoplankton bloom event, and the corresponding changes in bio-optical properties were tracked at one station (114.29°E, 22.06°N) located in the Pearl River estuary. A greater than 10-fold increase in POC (112.29-1173.36 mg m⁻³) was observed during the bloom, with the chlorophyll a concentration (Chl-a) varying from 0.984 to 25.941 mg m⁻³. A power law function is used to describe the relationship between POC and Chl-a, and the POC:Chl-a ratio tends to change inversely with Chl-a. Phytoplankton carbon concentration is indirectly estimated using the conceptual model proposed by Sathyendranath et al. (2009), and this carbon is found to contribute 47.21% (±10.65%) to total POC. The estimated carbon-to-chlorophyll ratio of phytoplankton in diatom-dominated waters is found to be comparable with results reported in the literature. Empirical algorithms for determining the concentrations of Chl-a and POC were developed based on the relationships of these variables with the blue-to-green reflectance ratio. With these bio-optical models, the levels of particulate organic carbon and Chl-a could be predicted from the radiometric data measured by a marine optical buoy, which showed much more detailed information about the variability in biogeochemical parameters during this bloom event.

[Progress in Teflon AF LWCC/LCW applications].

Teflon AF is chemically very inert, quite physically and optically stable, a highly vapor-permeable polymer with optical transparency through much of the UV-Vis region and with an RI lower than that of water, so Teflon AF LWCC/LCW (Long path-length liquid waveguide capillary cell/liquid core waveguides) has been used with a range of different detection techniques, including absorbance spectroscopy, fluorescence spectroscopy, Raman spectroscopy, and gas sensor. The present article describes the properties and the aspects of Teflon AF LWCC/LCW instrumentation and applications. And finally,the future prospect and outlook of Teflon AF LWCC/LCW is also discussed.